Journal: PLoS ONE

Article Title: Retinoid Signaling in Pancreatic Cancer, Injury and Regeneration

doi: 10.1371/journal.pone.0029075

Figure Lengend Snippet: (C) CRBP1 methylation status in PC cell lines and (D) restoration of CRBP1 expression with combination treatment of MiaPaCa2 cells with 5-Aza and TSA. (E) CRBP1 knockdown in stably transfected HPDE-EcoR cells. (F) HPDE cells grown in 3D demonstrate no change in morphology between siCRBP1 cells and scrambled control.

Article Snippet: Six pancreatic cancer cell lines were used: AsPC-1, BxPC-3, Capan-2, HPAC, MiaPaCa2 and PANC1 (ATCC, VA, USA).

Techniques: Methylation, Expressing, Knockdown, Stable Transfection, Transfection, Control

Journal: Cancer letters

Article Title: NEMO Peptide Inhibits the Growth of Pancreatic Ductal Adenocarcinoma by Blocking NF-κB Activation

doi: 10.1016/j.canlet.2017.09.018

Figure Lengend Snippet: A. Kras-induced constitutive NF-κB expression was measured by Western blot analysis in HPNE, HPNE/Vec, HPNE/KrasG12V, HPNE/KrasG12V/P16sh, MiaPaca2, and Colo357 cell lines; B. NF-κB expression levels in HPNE/KrasG12V, HPNE/KrasG12V/P16sh, MiaPaca2, and Colo357 cells were significantly higher than in HPNE and HPNE/Vec cells; C, Western blot analysis shows phosphorylated NF-κB expression in nuclear extract of HPNE/KrasG12V/P16sh cells treated with 50, 100, or200 μM NBDP; D, electrophoretic mobility shift assay shows phosphorylated NF-κB expression in HPNE/KrasG12V/P16sh cells treated with 50, 100, or 200 μM NBDP; E, Western blot analysis shows phosphorylated NF-κB expression in total cell lysates of HPNE/KrasG12V/P16sh cells treated with 50, 100, or200 μM NBDP; F and G, Western blot analysis shows phosphorylated NF-κB expression in total cell lysates of MiaPaca2 and Colo357 cells treated with 50, 100, or 200 μM NBDP. IOD: integrated optical density

Article Snippet: The human PDAC cell line MiaPaca2 was purchased from the American Type Culture Collection.

Techniques: Expressing, Western Blot, Electrophoretic Mobility Shift Assay

Journal: Cancer letters

Article Title: NEMO Peptide Inhibits the Growth of Pancreatic Ductal Adenocarcinoma by Blocking NF-κB Activation

doi: 10.1016/j.canlet.2017.09.018

Figure Lengend Snippet: A. Immunoprecipitation analysis showed that TNF-α (10 ng/mL) factor stimulated and promoted IKKγ to combine with IKKβ in HPNE/KrasG12V/P16sh cells; NBDP (100 μM) was competitive with IKKβ, decreasing the IKK γ combination under TNF-α (10 ng/mL) factor stimulation; B. Western blot analysis showed the change of NF-κB pathway in HPNE/KrasG12V/P16sh cells treated with NBDP (100 μM); C. Western blot analysis measured phosphorylated NF-κB expression in HPNE/KrasG12V/P16sh cells treated with gemcitabine (10 μM) in 0 h, 1 h, 2 h, 4 h, 6 h, 12 h; D. phosphorylated NF-κB expression was significantly increased in HPNE/KrasG12V and HPNE/KrasG12V/P16sh cells treated with gemcitabine at 2 h and 4 h (2 h vs 0 h, *P=0.0118; 4 h vs 0 h, **P=0.0029); E. Western blot analysis measured phosphorylated NF-κB expression in HPNE/KrasG12V/P16sh, MiaPaca2, and Colo357 cells treated with gemcitabine (10 μM) combined with NBDP (100 μM); F. NBDP decreased gemcitabine chemo-resistance due to increased phosphorylated NF-κB expressions in HPNE/KrasG12V/P16sh (gemcitabine vs control, *P=0.0001; combination vs gemcitabine, **P<0.0001), MiaPaca2 (gemcitabine vs control, *P<0.0001; combination vs gemcitabine, **P=0.0002), and Colo357 (gemcitabine vs control, *P=0.0014; combination vs gemcitabine, **P=0.0005) cell lines. Gem: gemcitabine; IOD: integrated optical density.

Article Snippet: The human PDAC cell line MiaPaca2 was purchased from the American Type Culture Collection.

Techniques: Immunoprecipitation, Western Blot, Expressing, Control

Journal: Cancer letters

Article Title: NEMO Peptide Inhibits the Growth of Pancreatic Ductal Adenocarcinoma by Blocking NF-κB Activation

doi: 10.1016/j.canlet.2017.09.018

Figure Lengend Snippet: A. MTT analysis showed that proliferation of HPNE/KrasG12V/P16sh, MiaPaca2, and Colo357 cells was inhibited by NBDP at doses of 5, 10, 25, 50, 100, 200, and 400 μM within 5 days; B. MTT analysis showed that proliferation of HPNE/KrasG12V/P16sh, MiaPaca2, and Colo357 cells was inhibited by gemcitabine at doses of 250 and 500 nM and 1, 5, 10, and 20 μM within 5 days; C. IC50 values of NBDP were detected by MTT analysis in HPNE/KrasG12V /P16sh, MiaPaca2, and Colo357 cells; D. MTT analysis showed that NBDP combined with gemcitabine inhibited proliferation in HPNE/KrasG12V/P16sh, MiaPaca2, and Colo357 cells. Ctrl: control; Gem: gemcitabine; IOD: integrated optical density.

Article Snippet: The human PDAC cell line MiaPaca2 was purchased from the American Type Culture Collection.

Techniques: Control

Journal: Cancer letters

Article Title: NEMO Peptide Inhibits the Growth of Pancreatic Ductal Adenocarcinoma by Blocking NF-κB Activation

doi: 10.1016/j.canlet.2017.09.018

Figure Lengend Snippet: A. Colony formation analysis showed that tumor cell proliferation was inhibited by NBDP at doses of 50, 100, and 200 μM in HPNE/KrasG12V/P16sh (50 μM NBDP vs control, *P=0.0004), MiaPaca2 (50 μM NBDP vs control, *P=0.0085), and Colo357 (50 μM NBDP vs control, *P=0.0218) cells; B. Colony formation analysis showed that NBDP treatment combined with gemcitabine inhibited proliferation of HPNE/KrasG12V/P16sh (NBDP vs control, *P=0.0123; combination vs gemcitabine, **P=0.0165), MiaPaca2 (NBDP vs control, *P=0.0114; combination vs gemcitabine, **P=0.0008), and Colo357 (NBDP vs control, *P=0. 0.0031; combination vs gemcitabine, **P=0.0026) cells; C. Wound-healing analysis showed that NBDP decreased invasion of HPNE/KrasG12V /P16sh, MiaPaca2, and Colo357 cells at doses of 50, 100, and 200 μM. Ctrl: control; Gem: gemcitabine

Article Snippet: The human PDAC cell line MiaPaca2 was purchased from the American Type Culture Collection.

Techniques: Control

Journal: Cancer letters

Article Title: NEMO Peptide Inhibits the Growth of Pancreatic Ductal Adenocarcinoma by Blocking NF-κB Activation

doi: 10.1016/j.canlet.2017.09.018

Figure Lengend Snippet: A. Flow cytometry analysis of the Annexin V staining assay showed the apoptosis of HPNE/KrasG12V/P16sh cells treated with NBDP at 50, 100, and 200 μM; B. the ratio of apoptosis increased with increasing NBDP doses in HPNE/KrasG12V/P16sh cells (50 μM NBDP vs control, *P=0.0004); C. Flow cytometry analysis showed that 100 μM NBDP combined with 5 μM gemcitabine increased apoptosis in HPNE/KrasG12V /P16sh, MiaPaca2, and Colo357 cells; D. The ratio of apoptosis increased with NBDP combined with gemcitabine in HPNE/KrasG12V/P16sh (NBDP vs control, *P=0.0870; combination vs gemcitabine, **P=0.0061), MiaPaca2 (NBDP vs control, *P=0.0055; combination vs gemcitabine, **P=0.0407), and Colo357 (NBDP vs control, *P=0.0659; combination vs gemcitabine, **P=0.0522) cells. Ctrl: control; Gem: gemcitabine

Article Snippet: The human PDAC cell line MiaPaca2 was purchased from the American Type Culture Collection.

Techniques: Flow Cytometry, Annexin V Staining Assay, Control

Journal: Cancer letters

Article Title: NEMO Peptide Inhibits the Growth of Pancreatic Ductal Adenocarcinoma by Blocking NF-κB Activation

doi: 10.1016/j.canlet.2017.09.018

Figure Lengend Snippet: A Western blot analysis showed the expression of Parp in HPNE/KrasG12V/P16sh cells treated with NBDP (50, 100, and 200 μM); B. The expression of Parp increased after NBDP treatment in HPNE/KrasG12V/P16sh cells (50 μM NBDP vs control, *P=0.0007); C. Western blot analysis showed the expression of cleaved-Parp and cleaved-caspase 3 in HPNE/KrasG12V/P16sh cells treated with NBDP (100 μM) combined with gemcitabine (10 μM); D–E. cleaved-Parp expression and cleaved-caspase 3 expression were both increased under the combination treatment with NBDP (100 μM) and gemcitabine (10 μM) in HPNE/KrasG12V/P16sh cells (C-Parp: combination vs gemcitabine, **P=0.0235; C-caspase 3: combination vs gemcitabine, **P=0.0075); F–G. MiaPaca2 and Colo357 cells were measured for expression of cleaved-Parp and cleaved-caspase 3 by Western blot analysis after the combination treatment with NBDP (100 μM) and gemcitabine (10 μM); H–K. cleaved-Parp expression and cleaved caspase 3 expression were both increased under the combination treatment with NBDP (100 μM) and gemcitabine (10 μM) in MiaPaca2 (C-Parp: combination vs gemcitabine, **P=0.0017; C-caspase 3: combination vs gemcitabine, **P=0.1033) and Colo357 (C-Parp: combination vs gemcitabine, **P=0.0585; C-caspase 3: gemcitabine vs gemcitabine, **P=0.0032) cells. Gem: gemcitabine; C: cleaved

Article Snippet: The human PDAC cell line MiaPaca2 was purchased from the American Type Culture Collection.

Techniques: Western Blot, Expressing, Control

Journal: Cancer letters

Article Title: NEMO Peptide Inhibits the Growth of Pancreatic Ductal Adenocarcinoma by Blocking NF-κB Activation

doi: 10.1016/j.canlet.2017.09.018

Figure Lengend Snippet: The NEMO-binding domain peptide (NBDP) of IKK was found to inhibit NF-κB activation in cancer cells. Mechanistic investigations showed that NBDP effectively competed with IKKγ for binding to IKKs and thus inhibited IKK/IκB/NF-kB pathway activation in PDAC.

Article Snippet: The human PDAC cell line MiaPaca2 was purchased from the American Type Culture Collection.

Techniques: Binding Assay, Activation Assay